Ideally, gene expression studies by quantitative RT-PCR (qRT-PCR) should be performed using RNA from homogeneous cell populations. Frequently, this means performing qRT-PCR using picogram amounts of ...
As molecular testing demands grow, there is a need to create stable, room-temperature polymerase chain reaction (PCR) assays that avoid cold chain storage and logistics, particularly in point-of-care ...
This is a preview. Log in through your library . Abstract The use of new powerful nested polymerase chain reaction (PCR) techniques to identify and screen for prevalence of parasites has a huge ...
High-resolution RNA analysis using next-generation sequencing (RNA-seq) offers a comprehensive assessment of the transcriptome, allowing for quantification of global gene expression. The utility of ...
Advanced therapy medicinal products (ATMPs) demand rigorous sterility testing. Yet traditional growth-based methods require 14 days for results, creating a critical bottleneck for short shelf-life ...
Figure 2: Tetraplasmic distribution of alleles is confirmed using pre-PCR separation by gel electrophoresis, which is not subject to PCR artifacts. We carried out two additional experiments to exclude ...
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